Prosensent effect of hydrogen sulfide in rat kidneys 

Ekaterina V. Trizno, Candidate of medical sciences, assosiate professor at the sub-department of pathological physiology, Astrakhan State Medical University (121 Bakinskaya street, Astrakhan, Russia), E-mail: neiron-2010@mail.ru 

Background. Hydrogen sulfide (H2S) in high concentrations is a toxic pollutant. Due to the risk of its impact on the organism of people living in the areas of its production as part of natural gas, it is necessary to expand the pathogenetically substantiated profile of diagnosing the consequences of its impact. The purpose of the study is to evaluate the effect of hydrogen sulfide (its sulfide ions H–HS) on the processes of senescence and peroxidation in the kidneys of old rats. Materials and methods: With the help of an H2S (Na2S) donor, intraperitoneal toxic effects of sulfide ions were simulated in 18-20-months-old rats. Sections and homogenates of renal tissue were examined for the presence of lipofuscincontaining senescent cells (SC), the level of interleukin-6, catalase, and oxidative modification of proteins in the presence of metals of variable valence. Results. SCs located diffusely between the renal bodies of the juxtamedullary and subcapsular nephrons of the kidney were identified. An increase in the level of interleukin-6, catalase and aldehyde dinitrophenylhydrazones was recorded. Conclusions. The applied experimental approach using Na2S as a donor promotes the formation of a senescent morphotype in a functioning kidney in old rats. The functional features of the kidneys determine the predominant toxic load on the renal corpuscles of the nephrons. Accumulation of lipofuscin by cells and an increase in the level of pro-inflammatory interleukin-6 are promoted by active processes of prooxidation of the protein components of cells. Distribution of the secretion of SA contributes to increased metabolic inconsistency and forms a vicious circle that increases the activity of the antioxidant catalase. 

Hydrogen sulfide, H2S, senescent cells, lipofuscin, kidney, interleukin-6, protein peroxidation 

Trizno E.V. Prosensent effect of hydrogen sulfide in murine kidneys. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Meditsinskie nauki = University proceedings. Volga region. Medical sciences. 2023;(4):221–230. (In Russ.). doi: 10.21685/2072-3032-2023-4-22